Our Technology
MEVs (Microalgae Extracellular Vesicles) offer striking opportunities in terms of suitable administration routes, and of a biodistribution profile that naturally targets key organs relevant to specific diseases.
MEVs are edible, intrinsically safe, easy to produce and to purify in large quantities, thus opening the way to address a large diversity of medical conditions by means of innovative therapeutics and vaccines.
Explore our Technology Platforms
EXO-loading MEV
MEV Characterisation in vitro
MEV Characterisation in vivo
Purified AGS’ MEV can be loaded by physical, scalable and industrializable methods, a process we call exo-loading.
Purified AGS’ MEVs can be loaded by physical methods, a process we call exo-loading.
Exo-loading is scalable and industrializable.
Native cell lines
Exogenous payload
AGS’ MEVs can be exo-loaded with a variety of molecules, varying in size, hydrophobicity, and nature, such as siRNA, mRNA, peptides, proteins, plasmids, oligonucleotides, or small molecules.
The biological activity of the exo-loaded payload is preserved, while at the same time it is protected from degradation by enzymes or other agents.
Loaded native MEVs
Besides the payload, neither the surface nor the behavior of the loaded MEV is altered by the endo-or the exo-loading.
From the outside, all AGS’ MEVs behave equally irrespective of whether they are empty, endo-loaded, or exo-loaded.
AGS’ MEVs, whether exo- or endo-loaded, can convey their payload to recipient cells of a myriad of origins, such as microalgae, bacteria, higher plant, mammalian, human.
Endo- or endo-loaded MEVs can also deliver the payload to the right cell compartments, ensuring the proper expression and biological activity of molecules having complex biological pathways such as siRNA, mRNA, receptor-binding peptides, among other.
Such a common external face, that is shared by all native MEVs, simplifies industrial and regulatory aspects, allows for the use of common downstream processing protocols, and leads to common PK and toxicity profiles (of the MEVs moiety) irrespective of the nature or diversity of the payloads.
MEV as Delivery Systems
ENDO-loading MEV
MEVs can also be loaded by the microalgae cells - a process we call endo-loading, before the MEVs are released to the culture medium.
Producer cell lines
Endo-loading requires the genetic engineering of the microalgae cells to make them express and internalize the heterologous payload inside the MEVs. Such engineered microalgae cells become producer cell lines, stable producers of endo-loaded MEVs.
MEVs producer cell lines can be handled (cloned, banked and stored) as it is routinely done for mammalian cell lines producers of therapeutic proteins.
AGS’ MEVs can be endo-loaded with heterologous molecules of interest such as siRNA, mRNA, peptides, or proteins.
Endo-loading of MEVs holds a great interest in terms of easiness, cost-effectiveness, and industrialization, compared to exo-loading approaches.
Endogenous payload
Large scale manufacturing - current capacity of 600L per batch
Product Design
Producer cell lines
Cell line engineering and cloning
Loaded native MEVs
Besides the payload, neither the surface nor the behavior of the loaded MEVs is altered by the endo-or the exo-loading.
From the outside, all AGS’ MEVs behave equally irrespective of whether they are empty, endo-loaded, or exo-loaded.
AGS’ MEVs, whether exo- or endo-loaded, can convey their payload to recipient cells of a myriad of origins, such as microalgae, bacteria, higher plant, mammalian, human.
Endo- or endo-loaded MEVs can also deliver the payload to the right cell compartments, ensuring the proper expression and biological activity of molecules having complex biological pathways such as siRNA, mRNA, receptor-binding peptides, among other.
Such a common external face, that is shared by all native MEVs, simplifies industrial and regulatory aspects, allows for the use of common downstream processing protocols, and leads to common PK and toxicity profiles (of the MEVs moiety) irrespective of the nature or diversity of the payloads.
The Manufacturing Platform is developed through AGS-M, a sister-company of AGS Therapeutics dedicated to process the development and manufacturing of MEVs and aimed at becoming a CDMO.
Loaded native MEV
Besides the payload, neither the surface nor the behavior of the loaded MEV is altered by the endo-or the exo-loading. Such loaded yet unmodified MEV are called native MEV.
Native MEV, whether exo- or endo-loaded, can convey their payload to recipient cells of a myriad of origins, such as microalgae, bacteria, higher plant, mammalian, human.
Native endo- or endo-loaded MEV can also deliver the payload to the right cell compartments, ensuring the proper expression and biological activity of molecules with complex biological pathways such as siRNA, mRNA, receptor-binding peptides.
From the outside, native MEV behave the same way irrespective of whether they are empty, endo-loaded, or exo-loaded.
Such common or generic outside face of the MEV significantly simplifies industrial and regulatory aspects such as the use of common downstream processing protocols, or a common PK and toxicity profile (of the MEV moiety) for MEV loaded with a diversity of payloads.
R&D programs
As dramatically seen with the current COVID-19 pandemic, therapeutics against viral and bacterial infections still represent a significant medical problem as new or resistant pathogens emerge. There is a vast, urgent, unmet need to bring rapid and adapted solutions against emerging diseases.
A common process for a variety of highly unique products
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